PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ANALYSIS OF AQUEOUS LEAF EXTRACT OF COFFEE SENNA (CASSIA OCCIDENTALIS LINN)Hadiza Abdullahi Sifawa, Kasimu Abubakar Shagari, Nasiru Modi, Hafiz Abubakar
Phytochemical screening and antibacterial analysis of aqueous leaf extract of Coffee sennawere carried out using standard biochemical procedures. Phytochemical screening of the extracts revealed the presence of alkaloids, tannis, steroids, volatile oils as well as saponin glycosides as the most abundant. Other phytochemicals identified in the extract were flavonoids, saponnins, and cardiac glycoside as moderately present. Balsam and terpenes were minutely present, while anthraquines was absent. Antibacterial screening of leaf extract of coffee senna(Cassia occidentalis) was carried out using agar well diffusion method for bacterial isolates(Bacillus subtilis, Escherichia coli, Salmonella paratyphi, Pseudomonas aeruginosa and Staphylococcus aureus). The result showed that the mean zones of inhibition were higher at 60 and 90 mg/ml against S. paratyphiandS.aureuswith 17.0 and 17.0 mm zones of inhibition respectively. The results showed that the extract of Cassia occidentaliscould be potent in inhibition of the studied microorganisms. Thus, the studied plant materials could be further investigated so as to validate their potent role in the treatment of infectious diseases caused by the tested microorganisms.
Keywords:Phytochemical, antibacterial, screening, leaf, Cassia occidentalis